Composite

Part:BBa_K1877001

Designed by: Ruijie Tang   Group: iGEM16_Gaston_Day   (2016-09-19)


pBAD + RBS + GFP + Single Terminator

This is the testing construct for arabinose inducible mechanism of 2016 Gaston Day School iGEM team's project, killswitch, which serves as a suicide program to prevent bacteria from escaping the laboratory environment and contaminating food and water system. It also serves as the construct, which helps us to determines the optimal concentration of arabinose of inducing the pBAD promoter. In response to arabinose concentration increase, the pBAD promoter should start the production of GFP. So, in a lab environment, like plates, the pBAD promoter should be continuously producing GFP. We test the production of GFP with fluorescences per OD600 through a spectrophotometer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 800


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Categories
Parameters
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